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Courses

 

Workshop: “Designing Molecular Tools for the Study of Integrative Physiology”

 

Córdoba, Argentina, December 5-7, 2016

 

 

 

Local Organizer: Laura Vivas (Instituto de Investigación Médica Mercedes y Martin Ferreyra, Córdoba, Argentina.)

Foreign Organizer: Willis K Samson (Saint Louis University School of Medicine, St. Louis, MO, USA).

Location: Instituto de Investigación Médica y Mercedes y Martin Ferreyra, Córdoba, Argentina. (INIMEC-CONICET-UNC). Friuli 2434, Córdoba, Argentina

Support: American Physiological Society, IBRO-LARC.

 

Description of Event:

The proposed Workshop will introduce pre- and postdoctoral fellows from Argentina and neighboring countries to the use of bioinformatics to identify novel gene sequences and to design/learn how to use affordable, molecular tools that the attendees can use to study Integrative Physiology. Participants will learn:

  • How to interrogate genome databases

  • Strategies for the validation of target sequences

  • Design of tools for gene compromise

  • Options for delivery of molecular tools

  • Strategies for the analysis of gene deletion or overexpression studies in vivo.

  • To analyze the “catFISH” imaging method by detecting the immediate early gene Arc(or Arg3.1), used as a neurophysiologic measure of spatial pattern separation.

  • To analyze brain double immunohistochemistry of Fos and Fluorogold to identify specific neuronal circuits.

The Workshop will include a combination of short lectures given by experts, followed by hands-on, computer driven, individual exercises in application of the target approaches. These will begin with assigned examples that the participants must interrogate and then lead to design exercises addressing their individual research projects. The goal of these exercises is not only to provide understanding of how to design and use modern molecular tools, but also why they are powerful tools and where/when those tools can provide novel insight into Integrative Physiology.

Faculty Details:

Willis K. Samson, Ph.D., D.Sc., FAPS

Professor of Pharmacology and Physiology

Saint Louis University School of Medicine

1402 South Grand Boulevard

St. Louis, MO 63104

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Laura M. Vivas, Ph.D.

Professor and Chief

Hydromineral Homeostasis Laboratory

Instituto de Investigación Médica Mercedes y Martín Ferreyra

University of Cordoba

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Gina L.C. Yosten, Ph.D.

Assistant Professor of Pharmacology and Physiology

Saint Louis University School of Medicine

1402 South Grand Boulevard

St. Louis, MO 63104

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Carol F. Elias, Ph.D.

Associate Professor of Molecular and Integrative Physiology

University of Michigan School of Medicine

1137 Catherine Street

7732B Med Sci II

Ann Arbor, MI 48109

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Víctor Ramírez-Amaya, Ph.D.

Professor of Neurobiology

Instituto de Neurobiología,

Universidad Nacional Autónoma de México.

Instituto de Investigación Médica Mercedes y Martín Ferreyr

 

Daniel C. Raimunda, Ph.D.

Assistant Professor

Instituto de Investigación Médica Mercedes y Martin Ferreyra

University of Cordoba

Cordoba, CP5016, Argentina

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Pablo Helguera, Ph.D.

Assistant Professor

Instituto de Investigación Médica Mercedes y Martin Ferreyra

University of Córdoba

Córdoba, CP5016, Argentina

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Carla Cisternas, Ph.D.

Assistant Professor

Instituto de Investigación Médica Mercedes y Martin Ferreyra

University of Cordoba

Cordoba, CP5016, Argentina

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Lauren M. Stein, Ph.D.

Postdoctoral Fellow in Pharmacology and Physiology

Saint Louis University School of Medicine

1402 South Grand Boulevard

St. Louis, MO 63104

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Program:

Day 1

0900 Registration

0930 Official Welcome and Introduction of Workshop Participants. Laura M. Vivas, Ph.D. Professor and Chief. Hydromineral Homeostasis Laboratory. Instituto de Investigación Médica Mercedes y Martín Ferreyra. University of Cordoba

1000 Lecture 1: The Power of Integrative Physiology: Using that Power to Discover and Publish. W.K. Samson Ph.D., D.Sc., F.A.P.S. Professor of Pharmacology and Physiology. Saint Louis University School of Medicine. Editor-in-Chief. The American Journal of Physiology. Regulatory, Integrative and Comparative Physiology

1100 Coffee Break

1130 Lecture 2: Interrogating the Gene Databases; The Basics of Bioinformatics. Gina L.C. Yosten, Ph.D. Assistant Professor of Pharmacology and Physiology. Saint Louis University School of Medicine

1230 Lunch Break

1400 Workshop Exer cise 1: Accessing the Databases (All participants will have access to internet and the NIH Databases)

 

1500 Workshop Exercise 2: Designing Primer Set for PCR (Students assigned target sequences for primer design.)

1600 Workshop Exercise 2 Continues with Participant Presentations of their Primer Designs

1700 Lecture 3: The Ins and Outs of qPCR. Gina L.C. Yosten, Ph.D. Assistant Professor of Pharmacology and Physiology. Saint Louis University School of Medicine

1800 Workshop Exercise 3: Statistical Analysis of qPCR Results (All Participants will be given raw data and expected to perform the analysis individually.)

Day 2

0900 Workshop Exercise 3 Continues with Participant Presentations of their analyses.

1000 Lecture 4: Silencing RNAs: Design and Application. Gina L.C. Yosten, Ph.D. Assistant Professor of Pharmacology and Physiology. Saint Louis University School of Medicine

1100 Coffee Break

1130 Workshop Exercise 4: Design your own RNAi (Participants will be assigned targets for RNAi design.)

1230 Lunch Break

1400 Workshop Exercise 4 Continues with Participant presentation of their constructs.

1500 Lecture 5: Proofing RNAi Effectiveness. Willis K. Samson, Ph.D., D.Sc., F.A.P.S. Professor of Pharmacology and Physiology. Saint Louis University School of Medicine

1600 Lecture 6: New Methods for Molecular Interrogation of Integrative Physiology (CRISPr). Carol F. Elias, Ph.D. Associate Professor of Molecular and Integrative Physiology. University of Michigan School of Medicine

1700 Coffee Break

1730 Workshop Exercise 5: Designing CRISPr/Caspase knockouts (Participants will be assigned target genes and required to design the targeting constructs).

Day 3

0900 Lecture 7: Validation of Gene Deletions and Strategies for Overexpression Rescue. Carol F. Elias, Ph.D. Associate Professor of Molecular and Integrative Physiology. University of Michigan School of Medicine

1000 Lecture 8: The use of “catFISH” imaging method by detecting the immediate early gene Arc(or Arg3.1), as a neurophysiologic measure of spatial pattern separation. Víctor Ramirez-Amaya, Ph.D. Professor Titular “A”. Neurobiología Conductual y Cognitiva Instituto de Neurobiología-Univ Nac. Autónoma de México. Instituto de Investigación Médica Mercedes y Martín Ferreyra

1100 Coffee Break

1130 Lecture 9: Analyzing brain double immunohistochemistry of Fos and Fluorogold to identify specific neuronal circuits. Laura M. Vivas, Ph.D.

1230 Lunch Break

1400 Workshop 6: Assigned problems of Integrative Physiology (Participants will work on groups of 3 to define a strategy for the use of these molecular tools for the study of their assigned “problem.”)

1500 Workshop 6 Concludes with Group Presentations

1700 Concluding remarks and distribution of participation certificates and feedback questionnaires. Laura M. Vivas, Ph.D. Professor and Chief Hydromineral Homeostasis Laboratory Instituto de Investigación Médica Mercedes y Martín Ferreyra. University of Cordoba.

What do we study?

The IMMF-INIMEC is an organization aimed at promoting scientific research in basic branches of biomedicine and training  of scientists and technicians. Scientists of the IMMF-INIMEC seek to understand the causes of some of the most common conditions and diseases.

 

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